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Rapidly advancing technology has made digital imaging within reach of
every microscopist. There are now so many digital cameras
available for microscopy, it's often difficult to choose the right one:
still cameras, video cameras, consumer cameras... At Martin Microscope Company, we spend lots of time testing
new digital cameras in order to be able to recommend the right camera
for each customer and application. With this in mind, we have developed the
following resources to help in selecting a digital camera for your
microscope. Select from the topics below, or just scroll down.
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CAMERA TYPES:
Dedicated Microscope Digital Still Cameras: Dedicated
microscope digital cameras typically cost more for less resolution, but
they have the advantage of being directly controlled by a computer.
They typically provide a better preview image, often with focusing aids,
and the ability to acquire the image directly into various imaging
software though a TWAIN or plug-in interface. At this time, most 2MP digital cameras output the full resolution image as the preview,
while higher MP cameras provide a lower resolution preview than the final
still image will be.
Consumer Hand Held Digital Still Cameras: We at Martin
Microscope Company got into the consumer camera arena quite early with the
development of our MM99 adapter in 1999, originally for the popular Sony
Mavica cameras. As far as we know, we were the first to make and
market a professional microscope adapter for a consumer digital camera. These
cameras offer high resolution still images for very low cost as
compared to dedicated microscope digital cameras. Consumer cameras
change very quickly with new models every year. Scientific cameras
just aren't able to keep up. So, consumer hand-held cameras are
recommended for routine micrography, especially as a replacement for
instant film. They are also recommended for their versatility, being useable
both on and off of the microscope, and for the ease with which they can be
moved from one microscope to another. The images are normally stored on
internal memory cards from which they must be downloaded to the
computer. This can present a problem if the image files need to be
named as they are saved, and also may be inconvenient if the images
need to be manipulated or measured immediately upon being captured.
The exception to this is the Canon line of digital cameras
that include PC/ Mac control software.
Digital Video Cameras: NEW INFO on HD VIDEO! For several
years now, we have recommend using a DV camcorder with our MM99
adapter as the easiest way to acquire digital video from a microscope.
With the introduction of the Sony HDR-HC1 High Definition Video
Camcorder for around $2,000.00, High Definition video
microscopy became affordable for the first time! We've tested our MM99 adapter
with this camcorder and now with the new HRD-HC9 on various microscopes with outstanding results.
Like other DV camcorders that we've used in the past, it can be controlled by a computer via
Firewire.
Recordings can be made to the internal DV tape, or captured directly into
a computer. However, we think the primary use of HD video
microscopy will be to enhance classroom displays. The move in recent
years from classroom TV monitors to video projectors has greatly increased
screen sizes, and meant that standard video microscopy often looks very
grainy on a large projection screen. Now, HD compatible projectors
and HDTV flat-screen monitors in conjunction with our MHC999PKG
HD video camera package make High Definition classroom displays a
great option. Download sample HD
Videos!
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OTHER CONSIDERATIONS:
Sensors: The heart of every digital
camera is the Sensor, usually either a CCD or a CMOS type. All
Sensors are analog devices, converting photons into electrical
signals. The process by which the analog information is changed
to digital is called Analog to Digital (A/D) Conversion. This
can occur within the Sensor itself, or within the camera, or within
the PC, as was the case with older video camera / frame grabber
technology. The Sensor size is another consideration. The
larger the Sensor size (actually the larger the individual pixel
size), the more light sensitive the Sensor should be, so larger is
better, at least in theory. Some of the most advanced digital
cameras, like the ProgRes C14+, use a large 1.4MP moving Sensor
that scans the image area in up to 36 increments to produce extremely
high resolution 12.5MP final images.
Resolution: The Sensor Resolution is the total number of picture elements (Pixels)
forming the image. Resolution ranges from 0.3 Megapixels (MP) for 640x480
analog video resolution up to an incredible 32MP with the new Lumenera
InfinityX-32. There is a 4x jump in
total pixels from 0.3MP to 1.3MP, roughly double the resolution both
horizontally and vertically (0.3 x 4 = 1.2), so we can see a vast
improvement in 1.3MP cameras over older video resolution cameras.
Images in this category are still relatively small in file size, so are
fine for email in jpg format. Next comes 2MP and 3.3MP cameras
which are less apparent improvements over 1.3MP. When we reach 5MP, that
represents 4x the total pixels of a 1.3MP camera (1.3 x 4 = 5.2), so 5MP is the
next big leap. Eight, ten, twelve MP cameras, again, are
improvements over 5MP cameras, but the next big 4x step requires a 20MP
camera which in most cases exceed the resolution of the
microscope optics. Indeed, we have experienced "empty
magnification" even in 8MP cameras. Generally, higher
megapixel images can provide greater detail over a wider field-of-view
(which makes them better for lower magnification imaging),
allow for cropping more detailed areas of interest, and can yield more
accurate measurements. They can also be used to make larger photo
quality (300dpi) prints. The following chart illustrates this:
| 0.3 Megapixel |
640 x 480 pixels |
1.6" x 2.13" print size at 300dpi |
Moticam 480 |
| 1.3 Megapixel |
1392 x 1040 pixels |
3.2" x 4.3" print size at 300dpi |
Lumenera Infinity2-1C |
| 2.0 Megapixel |
1600 x 1200 pixels |
4" x 5.3" print size at 300dpi |
Scion CFW-1608C |
| 3.3 Megapixel |
2080 x 1542 pixels |
5.1" x 6.8" print size at 300dpi |
ProgRes C3 |
| 5.0 Megapixel |
2580 x 1944 pixels |
6.4" x 8.5" print size at 300dpi |
ProgRes C5 |
| 6.0 Megapixel |
2816 x 2112 pixels |
9.4" x 7" print size at 300dpi |
Canon PowerShot S3IS |
| 8.0 Megapixel |
3264 x 2448 pixels |
8" x 11" print size at 300dpi |
Sony Cybershot H9 |
| 12.5 Megapixel |
4080 x 3072 pixels |
10.24 " x 13.6" at 300dpi |
ProgRes C14+ |
| 32 Megapixel |
6464 x 4864 pixels |
16" x 21.5" at 300dpi |
Lumenera InfinityX-32 |
Included interface software and compatibility: This is
an area that can play a very
important role in selecting the proper camera for a specific
application. This aspect will mainly impact dedicated microscope
cameras since consumer cameras usually do not directly interface with a
computer (other than to download captured images; Canon cameras are the
exception - see below). All dedicated
microscope cameras will include some software for driving the
camera. This will allow a preview image to be viewed on the
computer monitor which may be of the full camera resolution or may be a
reduced resolution for a faster refresh rate. The higher the
camera's pixel resolution, the more likely that it will have a reduced
resolution preview. We might consider this the camera's video
output capability, although the rate at which the preview is output will
depend primarily on the exposure time set for a given image, but also on
the speed of the computer processor, and the type of connection to the
computer (ie: Firewire, USB2, etc). Most higher
megapixel cameras, because of their lower resolution previews, will
offer focusing aids such as a small portion of the image (user defined)
at full resolution, or a focus indicator bar. Common menu controls
include exposure time, gain, color balance, and possibly time lapse
features. In many cases the camera can automatically adjust
exposure time, color balance, etc, but a manual override is critical for
many microscopy challenges. Other software features might include
basic screen calibration and measuring, more commonly on the captured
image, but sometimes on the "live" preview image as
well. Some cameras are compatible only with PC's, but most will
support Macs. Finally, basic TWAIN compatibility is
desired (for PCs) so that other software like Adobe Photoshop can access
the camera interface software and acquire images directly. Many
cameras also include plug-ins for popular image analysis
software. Our current recommended IA software is IMT i-Solution
which is compatible with our best dedicated microscopy
cameras.
FREQUENTLY ASKED QUESTION: What is the
magnification of the captured image? ANSWER...
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FEATURED CAMERAS:
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DEDICATED MICROSCOPE CAMERAS:
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| Camera lists
below do not include
the full product ranges of each manufacturer. Please visit the
manufacturers' websites linked below for more information on their full
offerings of digital microscope cameras. |
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Jenoptik ProgRes Professional Color CCD Firewire
Cameras:
- Made in Germany by Jenoptik Laser.Optic.Systeme,
GmbH
- Include ProgRes CapturePro software for Win/Mac
- Accept standard C-mount microscope adapters
Please visit www.progres-camera.com
for more information on these and additional models, or click the model name for more on that specific
model from Jenoptik. |
| Model |
Megapixels |
Sensor |
Recommended C-mount |
Pixel Size |
Output |
Price |
| CT3 |
3.2 |
1/2" CMOS 2048x1536 |
0.5x or 0.63x |
3.2um2 |
Firewire |
$2,490.00 |
| C3 Cooled |
3.3 |
1/2" CCD 2080x1542 |
0.5x or 0.63x |
3.45um2 |
Firewire |
$3,490.00 |
| C5 Cooled |
5.0 |
2/3" CCD 2580x1944 |
0.63x |
3.40um2 |
Firewire |
$4,490.00
Recommended |
| CF-Scan
Cooled |
12.5
(4080x3072) |
2/3" 9x Scanning CCD 1360x1024 |
0.63x |
6.45um2 |
Firewire |
$7,990.00 |
| C14Plus Cooled with True Color |
12.5
(4080x3072) |
2/3" 36x Scanning CCD 1360x1024 |
0.63x |
6.45um2 |
Firewire |
$9,990.00 |
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Lumenera Infinity Color CCD or CMOS USB-2
Cameras:
- Made in Canada by Lumenera Corporation
- Include Infinity Capture and Infinity Analyze for Windows only
- Accept standard C-mount microscope adapters (except for 4-11)
Please visit http://www.lumenera.com/scientific/index.php
for more information, or click the model name for more on that specific
model from Lumenera. |
| Model |
Megapixels |
Sensor |
Recommended C-mount |
Pixel Size |
Output |
Price |
| Infinity Lite |
1.5 |
1/2.5" CMOS 1440x1080 |
0.5x or 0.63x |
4.2um2 |
USB-2 |
$900.00 |
| Infinity1-5 |
5.0 |
1/2.5" CMOS 2592x1944 |
0.5x or 0.63x |
2.2um2 |
USB-2 |
$2,800.00 |
| Infinity2-1C |
1.4 |
1/2" CCD 1392x1040 |
0.5x or 0.63x |
4.65um2 |
USB-2 |
$1,695.00
Recommended |
| Infinity2-2C |
2.0 |
1/2" CCD 1616x1216 |
0.5x or 0.63x |
4.4um2 |
USB-2 |
$2,700.00 |
| Infinity2-3C |
3.3 |
1/1.8" CCD 2080x1536 |
0.5x or 0.63x |
3.45um2 |
USB-2 |
$3,000.00 |
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InfinityX-32 |
2, 4, 8, or 32
(6464x4864) |
1/1/8" Scanning CCD 1616x1216 |
0.5x or 0.63x |
4.4um2 |
USB-2 |
$5,500.00
Recommended |
Infinity4-11
Nikon F-mount |
10.7 |
35mm Large Format CCD 4008x2672 |
N/A |
9um2 |
USB-2 |
$8,500.00 |
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Scion CFW Color CCD Firewire Cameras:
- Made in USA by Scion Corporation
- Include Scion VisiCapture and ImageJ Software for Win/Mac
- Accept standard C-mount microscope adapters
Please visit http://www.scioncorp.com
for more information, or click the model name for more on that specific
model from Scion.
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| Model |
Megapixels |
Sensor |
Recommended C-mount |
Pixel Size |
Output |
Price |
| CFW-1308C |
1.3 |
1/2" CCD 1360x1024 |
0.5x or 0.63x |
4.65 |
Firewire |
$1,295.00 |
| CFW-1608C |
2.0 |
1/1.8" CCD 1600x1200 |
0.5x or 0.63x |
4.4 |
Firewire |
$1,895.00 |
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Motic Moticam Color CMOS USB-2 Cameras:
- Made in China by Motic
- Include Motic Images Plus Software for Win/Mac
- Accept standard C-mount microscope adapters
Please visit http://www.motic.com/Products.aspx?r=NA&lang=en&id=144
for more information on Motic cameras, or click the model name for more on
that specific model from Motic.
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| Model |
Megapixels |
Sensor |
Recommended C-mount |
Pixel Size |
Output |
Price |
| MC1000 |
1.3 |
1/2" CMOS 1280x1024 |
0.5x or 0.65x |
? |
USB-2 |
$399.00 |
| MC2000 |
2.0 |
1/2" CMOS 1600x1200 |
0.5x or 0.65x |
? |
USB-2 |
$699.00 |
| MC2300 |
3.0 |
1/2" CMOS 2048x1536 |
0.5x or 0.65x |
? |
USB-2 |
$999.00 |
DIGITAL CAMERAS:
(Consumer Hand-Held)
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May 1, 2008: New Camera
Packages!
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PRICE
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MXSISLRPKG 12.5 Megapixel DSLR Microscope Camera Package
- Canon EOS Digital Rebel XSi 12.5MP
DSLR Camera
- MM-SLR
Microscope Adapter (or MDSLR if available)
- T-Mount for Canon EOS
- AC Adapter
- 18-55mm Kit Lens (for
off-microscope use)
- 2 GB Secure Digital Card
This is the first DSLR camera we've tested that we really like for
microscopy. The included EOS Utility software with "Live
View" preview mode makes all the difference. More
on DSLR cameras...
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$1,399.00
Recommended
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MH1099PKG 8-Megapixel Digital Microscope Camera Package:
- Sony Cybershot H10 8MP Digital Camera
- MM99-58
Microscope Adapter
- AC Adapter
- 2 GB Memory Stick Duo ProThis
camera lacks the tilting LCD and software control that we like in the
Canon S5, but still
works quite well with the MM99 adapter. The Sony H50 has the tilting LCD, but its 15x zoom lens does not work well
with our adapters - too much pin-cushion, and problems with vignetting in
some circumstances. The previous model H9 worked well.
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$699.00
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MP5100PKG 12-Megapixel Digital Microscope Camera Package:
- Nikon Coolpix 5100 12.5MP Digital Camera
- MMC00L-E20
Microscope Adapter
- 2 GB Secure Digital Card
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$799.00
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HD DIGITAL VIDEO:
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Sample HD Video
Downloads...
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PRICE |
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MHC999PKG 1080i HD Digital Microscope Camcorder Package:
- Sony HDR-HC9 High Definition
Digital Camcorder
- MM99
Microscope Adapter
- FREE 1GB Memory Stick Duo Pro
- FREE USB2 Multi-Card Reader |
$1,399.00 |
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(microscope sold separately)
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MHC99S40 1080i HD Digital Microscope Camcorder
Package with 40" 1920x1080 HD Monitor:
- Sony HDR-HC9 High Definition
Digital Camcorder
- MM99
Microscope Adapter
- Sony KDL-40W4100 40" HDTV
Monitor 1920x1080 resolution
- FREE 1GB Memory Stick Duo Pro
- FREE USB2 Multi-Card Reader
- FREE HDMI Cable, 10' length
The W4100 HDTV included with this package tested best
among HDTV's we've tried so far, but other Sony HDTV monitors and projectors are also available -
see SonyStyle
for other models.
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$3,299.00 |
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XGA-CAM Digital Video Camera
This new camera is in a category of its
own. It outputs live 60fps HD video directly to a PC monitor or
projector at XGA resolution of 1024x768 pixels using a standard VGA
monitor cable. There's no capture capability for still or video, and
no computer required. The XGA-CAM is designed for live imaging
for classrooms and conferences at a resolution almost double that of
analog video. Requires a standard C-mount microscope adapter (sold
separately). |
$995.00 |
Image Processing and Analysis Software:
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IMT i-Solution Image Analysis Software:
Features manual and automatic measurements, fast multi-focus enhancement
(now with automatic stereo parallax correction), full image analysis
processing and thresholding. Includes drivers for Lumenera and
Jenoptik cameras and others; TWAIN compatible.
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$2,500.00
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| IMT i-Solution Lite
Software: Features manual measurements and fast multi-focus
enhancement (now with automatic stereo parallax correction).
Includes drivers for Jenoptik and Lumenera cameras and others; TWAIN.
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$1,000.00
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Download
IMT Software brochure...
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Synchroscopy Automontage
Essentials Multi-Focus Software:
The gold standard for fully-focused images, this basic version takes a
stack of serially numbered image files and combines them into one fully
focused image. Automatic parallax adjustment, three blending modes,
and other user adjustable parameters. Even includes 3D glasses!
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$999.00
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