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Rapidly advancing technology has made digital imaging
within reach of
every microscopist. There are now so many digital cameras
available for microscopy, it's often difficult to choose the right one:
still cameras, video cameras, consumer cameras... At Martin
Microscope Company, we spend lots of time testing
new digital cameras in order to be able to recommend the right camera
for each customer and application. That said, over the last five years,
we've realized that a few camera systems are consistent favorites and out-sell
all others by a wide margin.
Below are these best sellers - the systems our customers routinely choose and
that we stock most heavily. Click the cameras below for more information on these and
other similar cameras. View comparison images here...
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Camera Systems:
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Moticam 1000
Educational Camera |
M14
& M50
Industrial / Medical Camera Systems |
Jenoptik
ProgRes 5MP Research Microscopy Camera Systems |
MT3i
Consumer DSLR Systems |
| 1.3MP 1/3" CMOS USB |
1.4MP 1/2" CCD, USB /
5.0MP 2/3" CCD USB |
5MP 2/3" CCD, USB |
18MP CMOS, USB |
| Mfg by Motic, China |
Mfg by Lumenera, Canada |
Mfg by Jenoptik,
Germany |
Mfg by Canon, Japan,
and Martin Microscope, USA |
| May require C-mount |
Requires C-mount (0.5x
/0.6x) |
Requires C-mount (0.63x +) |
May require 1x C-mount |
| Windows XP, Vista, 7 |
Windows XP, Vista, 7 |
Windows XP, Vista, 7 |
Windows / Mac |
| from $399.00 |
from $1,695.00 |
from $4,490.00 |
from $1,499.00 |
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What's the
difference?
Dedicated Microscope
Digital Still Cameras: Dedicated
microscope digital cameras typically cost more for less resolution, but
they have the advantage of being directly controlled by a
computer.
They typically provide a better preview image, often with focusing
aids,
and the ability to acquire the image directly into various imaging
software though a TWAIN or plug-in interface. The consumer DSLR cameras
are catching up, though. Read on!
Consumer Hand Held
Digital Still Cameras: We at Martin
Microscope Company got into the consumer camera arena quite early with
the
development of our MM99 adapter in 1999, originally for the popular
Sony
Mavica cameras. As far as we know, we were the first to make
and
market a professional microscope adapter for a consumer digital
camera.
Since then, we've recommended consumer hand-held cameras for routine
micrography, especially as a replacement for
instant film. They are also recommended for their
versatility, being useable
both on and off of the microscope, and for the ease with which they can
be
moved from one microscope to another. The images are normally
stored on
internal memory cards from which they must be downloaded to the
computer. This can present a problem if the image files need
to be
named as they are saved, and also may be inconvenient if the
images
need to be manipulated or measured immediately upon being
captured.
The exception to this is the Canon line of digital cameras
that include PC/ Mac control software. Now,
in 2010, most consumer point-and-shoot digital cameras have been
cheapened to the point that they no longer have lens filter threads to
which to attach a microscope adapter, nor do they offer a tilting LCD
which is useful when the camera is mounted vertically.
The
DSLR cameras now dominate the consumer camera market, and of all the
DSLR's we've tested, the Canon EOS series is tops for microscopy (for
these reasons...).
Digital Video
Cameras: NEW INFO on HD VIDEO!
After five years of using, selling and recommending
Sony HD
camcorders, we've now changed our product recommendation to the new
Canon Vixia HF S30 camcorder for HD video (although the Sony
cameras also work fine - we mostly just don't like the placement of the
mini-HDMI output port on the new Sony HX550V). In our experience, the primary use of HD
video
microscopy has been to enhance classroom and conference room
displays. The move in recent
years from classroom TV monitors to video projectors has greatly
increased
screen sizes, and meant that standard video microscopy often looks very
grainy on a large projection screen. Now, HD compatible
projectors
and HDTV flat-screen monitors in conjunction with our MCV30
HD video camera package make High Definition displays
a
great option. View sample HD
Videos!
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Sensors:
The heart of every digital
camera is the Sensor, usually either a CCD or a CMOS type.
All
Sensors are analog devices, converting photons into electrical
signals. The process by which the analog information is
changed
to digital is called Analog to Digital (A/D) Conversion. This
can occur within the Sensor itself, or within the camera, or within
the PC, as was the case with older video camera / frame grabber
technology. The Sensor size is another
consideration. The
larger the Sensor size (actually the larger the individual pixel
size), the more light sensitive the Sensor should be, so larger is
better, at least in theory. Some of the most advanced digital
cameras, like the ProgRes C14+, use a large 1.4MP moving
Sensor
that scans the image area in up to 36 increments to produce extremely
high resolution 12.5MP final images.
Resolution:
The Sensor Resolution is the total number of picture elements (Pixels)
forming the image. Resolution ranges from 0.3 Megapixels (MP)
for 640x480
analog video resolution up to an incredible 32MP with the new Lumenera
InfinityX-32. There is a 4x jump in
total pixels from 0.3MP to 1.3MP, roughly double the resolution both
horizontally and vertically (0.3 x 4 = 1.2), so we can see a vast
improvement in 1.3MP cameras over older video resolution
cameras.
Images in this category are still relatively small in file size, so are
fine for email in jpg format. Next comes 2MP and 3.3MP
cameras
which are less apparent improvements over 1.3MP. When we
reach 5MP, that
represents 4x the total pixels of a 1.3MP camera (1.3 x 4 = 5.2), so
5MP is the
next big leap. This is why there are no 2MP or 3.3MP cameras in our top
choice cameras above. Eight, ten, twelve MP cameras, again, are
improvements over 5MP cameras, but the next big 4x step requires a 20MP
camera which in most cases exceed the resolution of the
microscope optics. Indeed, we have experienced "empty
magnification" even in 8MP cameras. Generally, higher
megapixel images can provide greater detail over a wider field-of-view
(which makes them better for lower magnification imaging),
allow for cropping more detailed areas of interest, and can yield more
accurate measurements. They can also be used to make larger
photo
quality (300dpi) prints. The following chart illustrates
this:
| 0.3 Megapixel |
640 x 480 pixels |
1.6" x 2.13" print size at
300dpi |
analog video cameras |
| 1.3 Megapixel |
1392 x 1040 pixels |
3.2" x 4.3" print size at
300dpi |
M14 |
| 2.0 Megapixel |
1616 x 1216 pixels |
4" x 5.3" print size at
300dpi |
Lumenera Infinity2-2C |
| 3.3 Megapixel |
2080 x 1542 pixels |
5.1" x 6.8" print size at
300dpi |
Jenoptik ProgRes C3 |
| 5.0 Megapixel |
2448 x 2048 pixels |
6.4" x 8.5" print size at
300dpi |
M50 |
| 12.5 Megapixel |
4080 x 3072 pixels |
10.24 " x 13.6" at 300dpi |
Jenoptik ProgRes C14+ |
| 18 Megapixel |
5184 x 3456 pixels |
17.28" x 11.5" at 300dpi |
MT3i |
| 32 Megapixel |
6464 x 4864 pixels |
16" x 21.5" at 300dpi |
Lumenera InfinityX-32 |
Included interface
software and compatibility: This is
an area that can play a very
important role in selecting the proper camera for a specific
application. This aspect will mainly impact dedicated
microscope
cameras since consumer cameras usually do not directly interface with a
computer (other than to download captured images; Canon cameras are the
exception). All dedicated
microscope cameras will include some software for driving the
camera. This will allow a preview image to be viewed on the
computer monitor which may be of the full camera resolution or may be a
reduced resolution for a faster refresh rate. The higher the
camera's pixel resolution, the more likely that it will have a reduced
resolution preview. We might consider this the camera's video
output capability, although the rate at which the preview is output
will
depend primarily on the exposure time set for a given image, but also
on
the speed of the computer processor, and the type of connection to the
computer (ie: Firewire, USB2, etc). Most higher
megapixel cameras, because of their lower resolution previews, will
offer focusing aids such as a small portion of the image (user defined)
at full resolution, or a focus indicator bar. Common menu
controls
include exposure time, gain, color balance, and possibly time lapse
features. In many cases the camera can automatically adjust
exposure time, color balance, etc, but a manual override is critical
for
many microscopy challenges. Other software features might
include
basic screen calibration and measuring, more commonly on the captured
image, but sometimes on the "live" preview image as
well. Some cameras are compatible only with PC's, but most
will
support Macs. Finally, basic TWAIN compatibility is
desired (for PCs) so that other software like Adobe Photoshop can
access
the camera interface software and acquire images directly.
Many
cameras also include plug-ins for popular image analysis
software.
Our recommended Image Analysis software is IMT i-Solution
which is compatible with our M14, M50,
Jenoptik ProgRes, and MT3i camera
systems for Windows XP, Vista, and 7. IMT software is available in three
versions: i-Solution Capture; i-Solution
Lite; i-Solution Full
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FREQUENTLY ASKED
QUESTION: What is the
magnification of the captured image? ANSWER...
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